Journal: Frontiers in Genetics

Article Title: CircARVCF Contributes to Cisplatin Resistance in Gastric Cancer by Altering miR-1205 and FGFR1

doi: 10.3389/fgene.2021.767590

Figure Lengend Snippet: CircARVCF knockdown suppressed DDP resistance in DDP-resistant GC cells. (A) The expression of circARVCF in MKN-45/DDP and AGS/DDP cells transfected with si-circARVCF#1, si-circARVCF#2, si-circARVCF#3, or si-NC was detected by qRT-PCR. (B–I) MKN-45/DDP and AGS/DDP cells were transfected with si-NC or si-circARVCF#1. (B,C) IC50 of cisplatin in MKN-45/DDP and AGS/DDP cells was examined with CCK-8 assay. (D) The colony formation of MKN-45/DDP and AGS/DDP cells was evaluated by colony formation assay. (E,F) The migration and invasion of MKN-45/DDP and AGS/DDP cells were tested by transwell assay. (G) The apoptosis of MKN-45/DDP and AGS/DDP cells was analyzed by flow cytometry analysis. (H,I) The protein levels of MRP1, MDR1, Bcl-2 and Bax in MKN-45/DDP and AGS/DDP cells were measured via western blot assay. * p < 0.05.

Article Snippet: The antibodies included multidrug resistance protein 1 (MRP1; bs-24241R; 1:2000; Bioss), p-glycoprotein (MDR1; bs-0563R; 1:2000; Bioss), B-cell lymphoma-2 (Bcl-2; bs-20351R; 1:2000; Bioss), BCL2-Associated X (Bax; bs-28034R; 1:2000; Bioss), FGFR1 (bs-0230R; 1:2000; Bioss) and β-actin (bs-0061R; 1:10,000; Bioss).

Techniques: Expressing, Transfection, Quantitative RT-PCR, CCK-8 Assay, Colony Assay, Migration, Transwell Assay, Flow Cytometry, Western Blot

Journal: Frontiers in Genetics

Article Title: CircARVCF Contributes to Cisplatin Resistance in Gastric Cancer by Altering miR-1205 and FGFR1

doi: 10.3389/fgene.2021.767590

Figure Lengend Snippet: Inhibition of miR-1205 reversed the effects of circARVCF knockdown on DDP resistance, cell colony formation, migration, invasion, and apoptosis in DDP-resistant GC cells. MKN-45/DDP and AGS/DDP cells were transfected with si-NC, si-circARVCF#1, si-circARVCF#1+in-miR-NC, or si-circARVCF#1+in-miR-1205. (A,B) IC50 of cisplatin was estimated by CCK-8 assay. (C) The colony formation of MKN-45/DDP and AGS/DDP cells was examined with colony formation assay. (D,E) The migration and invasion of MKN-45/DDP and AGS/DDP cells were assessed by transwell assay. (F) The apoptosis of MKN-45/DDP and AGS/DDP cells was analyzed by flow cytometry analysis. (G,H) The protein levels of MRP1, MDR1, Bcl-2 and Bax in MKN-45/DDP and AGS/DDP cells were measured via western blot assay. * p < 0.05.

Article Snippet: The antibodies included multidrug resistance protein 1 (MRP1; bs-24241R; 1:2000; Bioss), p-glycoprotein (MDR1; bs-0563R; 1:2000; Bioss), B-cell lymphoma-2 (Bcl-2; bs-20351R; 1:2000; Bioss), BCL2-Associated X (Bax; bs-28034R; 1:2000; Bioss), FGFR1 (bs-0230R; 1:2000; Bioss) and β-actin (bs-0061R; 1:10,000; Bioss).

Techniques: Inhibition, Migration, Transfection, CCK-8 Assay, Colony Assay, Transwell Assay, Flow Cytometry, Western Blot

Journal: Frontiers in Genetics

Article Title: CircARVCF Contributes to Cisplatin Resistance in Gastric Cancer by Altering miR-1205 and FGFR1

doi: 10.3389/fgene.2021.767590

Figure Lengend Snippet: MiR-1205 regulated the DDP resistance and malignant behaviors of DDP-resistant GC cells by targeting FGFR1. MKN-45/DDP and AGS/DDP cells were transfected with miR-NC, miR-1205, miR-1205 + pcDNA or miR-1205 + FGFR1. (A,B) DDP resistance was analyzed by CCK-8 assay. (C–F) The colony formation, migration, invasion and apoptosis of MKN-45/DDP and AGS/DDP cells were evaluated by colony formation assay, transwell assay and flow cytometry analysis, respectively. (G,H) The protein levels of MRP1, MDR1, Bcl-2 and Bax in MKN-45/DDP and AGS/DDP cells were measured via western blot assay. * p < 0.05.

Article Snippet: The antibodies included multidrug resistance protein 1 (MRP1; bs-24241R; 1:2000; Bioss), p-glycoprotein (MDR1; bs-0563R; 1:2000; Bioss), B-cell lymphoma-2 (Bcl-2; bs-20351R; 1:2000; Bioss), BCL2-Associated X (Bax; bs-28034R; 1:2000; Bioss), FGFR1 (bs-0230R; 1:2000; Bioss) and β-actin (bs-0061R; 1:10,000; Bioss).

Techniques: Transfection, CCK-8 Assay, Migration, Colony Assay, Transwell Assay, Flow Cytometry, Western Blot

Journal: Bioorganic & medicinal chemistry letters

Article Title: Photoactivated 2,3-Distyrylindoles Kill Multi-Drug Resistant Bacteria

doi: 10.1016/j.bmcl.2018.04.001

Figure Lengend Snippet: Compound 1 eradicates MRSA (ATCC BAA 44) in the presence of light. A: Graph, showing a dose-dependent effect of 1 with and without a 2-minute irradiation with white light. B. Drip-streak plates utilized in the experiment illustrating the CFU counts. The data are derived from a single experiment performed in 3 replicates and serve as an example of 3 independent experiments producing similar results. The stars represent no observable colonies.

Article Snippet: The panel included: another multidrug-resistant MRSA strain (ATCC BAA1717, CA-MRSA); drug-sensitive Staphylococcus aureus ; vancomycin-resistant Enterococcus faecalis , known as VRE and representing another bacterial genus on the WHO list as discussed above; 26 Streptococcus pyogenes , a causative agent of important human diseases, ranging from superficial skin infections to life-threatening systemic infections; 27 Streptococcus mutans , a species commonly found in human oral cavity and the main contributor to tooth decay.

Techniques: Irradiation, Derivative Assay

Journal: Bioorganic & medicinal chemistry letters

Article Title: Photoactivated 2,3-Distyrylindoles Kill Multi-Drug Resistant Bacteria

doi: 10.1016/j.bmcl.2018.04.001

Figure Lengend Snippet: Bacterial patterning with light. Left: MRSA (ATCC BAA-44) cells pre-treated with 1 (1 μM), spread over an agar plate and irradiated with light through a mask for 2 minutes, and then incubated at 37 °C. NMT: New Mexico Tech. Right: Schematic of the bacterial patterning experiment.

Article Snippet: The panel included: another multidrug-resistant MRSA strain (ATCC BAA1717, CA-MRSA); drug-sensitive Staphylococcus aureus ; vancomycin-resistant Enterococcus faecalis , known as VRE and representing another bacterial genus on the WHO list as discussed above; 26 Streptococcus pyogenes , a causative agent of important human diseases, ranging from superficial skin infections to life-threatening systemic infections; 27 Streptococcus mutans , a species commonly found in human oral cavity and the main contributor to tooth decay.

Techniques: Irradiation, Incubation

Journal: Bioorganic & medicinal chemistry letters

Article Title: Photoactivated 2,3-Distyrylindoles Kill Multi-Drug Resistant Bacteria

doi: 10.1016/j.bmcl.2018.04.001

Figure Lengend Snippet: Compound 1 is effective against a variety of drug resistant Gram-positive bacterial strains in the presence of light. A. MRSA (ATCC BAA1717). B. S. aureus (ATCC 29213). C. E. faecalis (ATCC 51299). D. S. pyogenes (ATCC 8133). E. S. mutans (Ward’s 85W 2357). The data are derived from a single experiment performed in 3 replicates and serve as an example of 3 independent experiments producing similar results. The stars represent no observable colonies.

Article Snippet: The panel included: another multidrug-resistant MRSA strain (ATCC BAA1717, CA-MRSA); drug-sensitive Staphylococcus aureus ; vancomycin-resistant Enterococcus faecalis , known as VRE and representing another bacterial genus on the WHO list as discussed above; 26 Streptococcus pyogenes , a causative agent of important human diseases, ranging from superficial skin infections to life-threatening systemic infections; 27 Streptococcus mutans , a species commonly found in human oral cavity and the main contributor to tooth decay.

Techniques: Derivative Assay

Journal: Bioorganic & medicinal chemistry letters

Article Title: Photoactivated 2,3-Distyrylindoles Kill Multi-Drug Resistant Bacteria

doi: 10.1016/j.bmcl.2018.04.001

Figure Lengend Snippet: Eradication of MRSA (ATCC BAA44) by analogues of 1. The data are derived from a single experiment performed in 3 replicates and serve as an example of 3 independent experiments producing similar results. The stars represent no observable colonies.

Article Snippet: The panel included: another multidrug-resistant MRSA strain (ATCC BAA1717, CA-MRSA); drug-sensitive Staphylococcus aureus ; vancomycin-resistant Enterococcus faecalis , known as VRE and representing another bacterial genus on the WHO list as discussed above; 26 Streptococcus pyogenes , a causative agent of important human diseases, ranging from superficial skin infections to life-threatening systemic infections; 27 Streptococcus mutans , a species commonly found in human oral cavity and the main contributor to tooth decay.

Techniques: Analogues, Derivative Assay

Journal: Bioorganic & medicinal chemistry letters

Article Title: Photoactivated 2,3-Distyrylindoles Kill Multi-Drug Resistant Bacteria

doi: 10.1016/j.bmcl.2018.04.001

Figure Lengend Snippet: SEM images of MRSA (ATCC BAA44) cells treated with 1.

Article Snippet: The panel included: another multidrug-resistant MRSA strain (ATCC BAA1717, CA-MRSA); drug-sensitive Staphylococcus aureus ; vancomycin-resistant Enterococcus faecalis , known as VRE and representing another bacterial genus on the WHO list as discussed above; 26 Streptococcus pyogenes , a causative agent of important human diseases, ranging from superficial skin infections to life-threatening systemic infections; 27 Streptococcus mutans , a species commonly found in human oral cavity and the main contributor to tooth decay.

Techniques:

Journal: Bioorganic & medicinal chemistry letters

Article Title: Photoactivated 2,3-Distyrylindoles Kill Multi-Drug Resistant Bacteria

doi: 10.1016/j.bmcl.2018.04.001

Figure Lengend Snippet: Permeability of MRSA (ATCC BAA44) cell membrane probed by PI. Green fluorescence is due to 1. Red fluorescence is indicative of the disruption of cell membrane.

Article Snippet: The panel included: another multidrug-resistant MRSA strain (ATCC BAA1717, CA-MRSA); drug-sensitive Staphylococcus aureus ; vancomycin-resistant Enterococcus faecalis , known as VRE and representing another bacterial genus on the WHO list as discussed above; 26 Streptococcus pyogenes , a causative agent of important human diseases, ranging from superficial skin infections to life-threatening systemic infections; 27 Streptococcus mutans , a species commonly found in human oral cavity and the main contributor to tooth decay.

Techniques: Permeability, Membrane, Fluorescence, Disruption

Journal: Cancer Science

Article Title: Bmi‐1 gene is upregulated in early‐stage hepatocellular carcinoma and correlates with ATP‐binding cassette transporter B1 expression

doi: 10.1111/j.1349-7006.2009.01431.x

Figure Lengend Snippet: Overexpression and silencing of Bmi‐1 expression affected ATP‐binding cassette transporter B1 (ABCB1) expression in primary fetal hepatocytes and a hepatocellular carcinoma (HCC) cell line. (A) Bmi‐1 overexpression in primary fetal hepatocytes resulted in increased ABCB1 expression, compared with the mock‐transduced control (*P < 0.01; ***P = 0.038). (B) Silencing of Bmi‐1 expression by two different siRNAs (#1 and #2) in KIM‐1 cells was followed by a decrease in ABCB1 expression (*P < 0.01; **P = 0.08). Error bars were derived from three independent experiments.

Article Snippet: These were heated at 120°C in 0.01 mol/L sodium citrate buffer, pH 7.0, for 10 min before incubation with a mouse Bmi‐1 antibody (1/200; Upstate Biotechnology, Lake Placid, NY, USA) and a multidrug resistance protein 1 (MDR1) antibody (1/200; Santa Cruz Biotechnology, Santa Cruz, CA, USA).

Techniques: Over Expression, Expressing, Binding Assay, Control, Derivative Assay

Journal: Cancer Science

Article Title: Bmi‐1 gene is upregulated in early‐stage hepatocellular carcinoma and correlates with ATP‐binding cassette transporter B1 expression

doi: 10.1111/j.1349-7006.2009.01431.x

Figure Lengend Snippet: Correlation and immunostaining of Bmi‐1 and ATP‐binding cassette transporter B1 (ABCB1) expression in hepatocellular carcinoma (HCC). (A and B) Evaluation of Bmi‐1 and ABCB1 mRNA expression in HCC cell lines and HCC clinical samples. A strong correlation between Bmi‐1 and ABCB1 expression was observed in HCC cell lines and clinical samples by the Pearson’s correlation coefficient test (0.95, P = 0.01; and 0.90, P < 0.01, respectively) (black column, Bmi‐1; gray column, ABCB1). (c) Bmi‐1 and ABCB1 expression in early, moderately, and poorly differentiated HCC (magnification, ×200). Clear staining of Bmi‐1 “dot‐pattern” (scored as 2+), and a canalicular and cytoplasmic ABCB1 staining pattern (scored as 2+), was observed in well differentiated HCC. Bmi‐1 expression appeared weaker (scored as 1+), and only a canalicular staining pattern of ABCB1 (scored as 1+), was seen in moderately differentiated HCC. No dot‐pattern of Bmi‐1 and an absence of ABCB1 staining were observed in poorly differentiated HCC (scored as negative). Both Bmi‐1 and ABCB1 expression decreased with the progression of HCC, suggesting their correlated expression.

Article Snippet: These were heated at 120°C in 0.01 mol/L sodium citrate buffer, pH 7.0, for 10 min before incubation with a mouse Bmi‐1 antibody (1/200; Upstate Biotechnology, Lake Placid, NY, USA) and a multidrug resistance protein 1 (MDR1) antibody (1/200; Santa Cruz Biotechnology, Santa Cruz, CA, USA).

Techniques: Immunostaining, Binding Assay, Expressing, Staining

Journal: Cancer Science

Article Title: Bmi‐1 gene is upregulated in early‐stage hepatocellular carcinoma and correlates with ATP‐binding cassette transporter B1 expression

doi: 10.1111/j.1349-7006.2009.01431.x

Figure Lengend Snippet: Combined immunohistochemical analysis of Bmi‐1 and ATP‐binding cassette transporter B1 (ABCB1) expression in hepatocellular carcinoma

Article Snippet: These were heated at 120°C in 0.01 mol/L sodium citrate buffer, pH 7.0, for 10 min before incubation with a mouse Bmi‐1 antibody (1/200; Upstate Biotechnology, Lake Placid, NY, USA) and a multidrug resistance protein 1 (MDR1) antibody (1/200; Santa Cruz Biotechnology, Santa Cruz, CA, USA).

Techniques: Immunohistochemical staining, Expressing, Staining